Background:

The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. This gene is expressed in the brain or in neuronal tissue. An allelic variant encoding amino acid Lys at 915, instead of conserved Glu, has been identified in patients with mild intellectual disability.

Applications:

ELISA, WB, IHC

Name of antibody:

MCM3AP

Immunogen:

Fusion protein of human MCM3AP

Full name:

minichromosome maintenance complex component 3 associated protein

Synonyms：

GANP; SAC3; MAP80

SwissProt:

O60318

ELISA Recommended dilution:

2000-5000

IHC positive control:

Human colon cancer and human thyroid cancer

IHC Recommend dilution:

50-200

WB Predicted band size:

218 kDa

WB Positive control:

Hela and 293T cells

WB Recommended dilution:

200-1000