Background:

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene.

Applications:

ELISA, WB, IHC

Name of antibody:

MBD2

Immunogen:

Synthetic peptide of human MBD2

Full name:

methyl-CpG binding domain protein 2

Synonyms:

DMTase; NY-CO-41

SwissProt:

Q9UBB5

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human esophagus cancer and Human ovarian cancer

IHC Recommend dilution:

30-150

WB Predicted band size:

43 kDa

WB Positive control:

Human cerebrum tissue and Mouse brain tissue lysates

WB Recommended dilution:

200-1000